Basics in Flow Cytometry

We are excited to invite you to our upcoming "Basics in Flow Cytometry" workshop at OLS OMNI Life Science. The program will cover a range of topics, including instrument setup, sample preparation, data acquisition and analysis, as well as troubleshooting. Whether you are new to flow cytometry or are looking to refresh your skills, this training program is the perfect opportunity to enhance your knowledge and improve your research capabilities.

Topics of the Course

Basics

• Technical basics of Flow Cytometry: Structure and operation of the device
• Optical components and detectors: Laser, filters, and photomultiplier
• Fluorescence and scatter measurements: What is measured?
• Spillover - Spectral overlap
• Compensation: What is compensation and why is it important?
• Data analysis: Visualization and gating
• Creating compensation controls and adjusting compensation matrices
• Experiment design: What to consider when planning an experiment?
• Controls: How are positive and negative controls used?
• What controls are there? Isotype controls, FMO controls.

Applications

• Phenotyping: Identification of cell types based on surface markers
• Microvesicles: What are they and how are they analyzed?
• Apoptosis: How is cell death analyzed using Flow Cytometry?

Practical part

• Technical features of the NovoCyte Quanteon
• Instrument QC and Troubleshooting
• PBMC Staining
• Bacteria Staining
• Apoptosis
  

Goals

This course targets people working in a laboratory who are currently not familiar with flow cytometry. Participants of this course will be introduced or re-introduced to this topic and their knowledge on basic and special applications will be built up to a solid basis at the end. Requirement is the familiarity with conventional workflows and principles of cell culture.

Date and Pricing

Location: Bremen
Course language: English
Date: November 21 09:00 - November 22 16:30 2023
Price: 1.600 Euro ex. MwSt. (1.904 incl. MwSt.)

The course fee includes catering during the course (coffee, drinks, lunch) as well as any training material such as prints and a participation certificate after completion.