[AL192] Medium 199 was the first nutritionally defined medium developed by Morgan, Morton, and Parker in 1950 for nutritional studies on primary chick embryo fibroblasts in the absence of any additives. Media 199 are presently used for the maintenance of non-transformed cells, vaccine and virus production and primary explants of epithelial cells. AL192 is 10X concentrate with Hank's salts. It does not contain L-glutamine and sodium bicarbonate.

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[AL196] Ham's Nutrient Mixture F10 was designed for clonal growth of CHO cells and chick embryo cells under serum free conditions. It is now widely used for culturing a variety of cells including human diploid cells and white blood cells for chromosomal analysis and primary explants of rat, rabbit and chicken tissues. AL196 contains 20mM HEPES buffer and does not contain L-glutamine and sodium bicarbonate.

[AL197A] RPMI 1640 medium is extensively used for a wide range of anchorage dependant cell lines. The medium is known to support growth of cells both in presence of 5-20% FBS and in absence of FBS. AL197A is supplemented with 20mM HEPES buffer and L-glutamine. It does not contain sodium bicarbonate.

[AL198] RPMI 1640 medium is extensively used for a wide range of anchorage dependant cell lines. The medium is known to support growth of cells both in presence of 5-20% FBS and in absence of FBS. AL198 is supplemented with 20mM HEPES buffer and 1gm per litre sodium bicarbonate. It does not contain L-glutamine.

[AL199A] RPMI 1640 medium is extensively used for a wide range of anchorage dependant cell lines. The medium is known to support growth of cells both in presence of 5-20% FBS and in absence of FBS. AL199A contains 1mM sodium pyruvate, 2mM L-glutamine, 4.5gms per litre glucose, 2.0gms per litre sodium bicarbonate and does not contain HEPES buffer.

[AL199S] RPMI 1640 medium is extensively used for a wide range of anchorage dependant cell lines. The medium is known to support growth of cells both in presence of 5-20% FBS and in absence of FBS. AL199S contains 1mM sodium pyruvate, 2mM L-glutamine, 4.5gms per litre glucose, 1.5gms per litre sodium bicarbonate and does not contain HEPES buffer.

[AL200A] RPMI 1640 medium is extensively used for a wide range of anchorage dependant cell lines. The medium is known to support growth of cells both in presence of 5-20% FBS and in absence of FBS. AL200A contains L-glutamine, 2gms per litre glucose and 0.165 moles per litre MOPS buffer. This medium is used as a diluent for antifungal agents that are water-soluble as well as water-insoluble.

[AL201A] RPMI 1640 medium is extensively used for a wide range of anchorage dependant cell lines. The medium is known to support growth of cells both in presence of 5-20% FBS and in absence of FBS. AL201A contains L-glutamine, 25mM HEPES buffer and 2.2gms per litre sodium bicarbonate.

[AL204A] Leibovitz’s Medium was specifically designed to grow cells in a CO2 free atmosphere. The medium can be used to grow human tumor cells and embryonic cells and also established cell lines like HeLa and Hep-2. The medium is frequently used in diagnostic virology where tissue cell lines or strains need to be grown in closed systems. Leibovitz’s medium obviates the need of frequent medium change. AL204A contains L-glutamine and sodium bicarbonate. It does not contain phenol red and sodium pyruvate.

[AL206A] HybridoXL™ Medium is a medium specially developed for propagation of hybridomas and other fastidious cell lines. It is a modified mixture of Dulbecco's Modified Eagle Medium and NCTC 135 supplemented with insulin. On supplementation with 10% fetal bovine serum it supports rapid growth of many hybridoma cell lines. AL206A contains L-glutamine, HEPES buffer, insulin and sodium bicarbonate.

[AL209A] Medium 199 was the first nutritionally defined medium developed by Morgan, Morton, and Parker in 1950 for nutritional studies on primary chick embryo fibroblasts in the absence of any additives. The medium when supplemented with serum can be used for growth of a wide variety of cells. Media 199 are presently used for the maintenance of non-transformed cells, vaccine and virus production and primary explants of epithelial cells. AL209A contains Hank's salts, 25mM HEPES buffer, L-glutamine and sodium bicarbonate.

[AL214A] RPMI 1640 medium is extensively used for a wide range of anchorage dependant cell lines. The medium is known to support growth of cells both in presence of 5-20% FBS and in absence of FBS. AL214A is modified RPMI-1640 medium supplemented with 3.05mM L-glutamine, 10mM HEPES buffer, 2gms per litre sodium bicarbonate.

[AL215A] Dulbecco’s Modified Eagle Medium / Nutrient Mixture F12 Ham (DMEM/F12, 1:1 mixture) was originally formulated for rat neuroblastoma cells and MDCK cells.AL215A is DMEM/Nutrient Mixture F-12 Ham with L-glutamine, sodium bicarbonate and 15mM HEPES buffer. It does not contain phenol red and trace elements elements.

[AL219A] DMEM is a modification of Basal Medium Eagle (BME) that contains four fold concentration of amino acids and vitamins. Dulbecco's Modified Eagle Medium contains 4.5gms glucose per litre, L-glutamine, sodium bicarbonate and sodium pyruvate. It does not contain calcium chloride and hence can be used for culture of suspension cells.

[AL221A] Minimum Essential Medium(MEM) was developed by Harry Eagle to meet the specific nutritional requirements of certain subtypes of HeLa cells and normal mammalian fibroblasts. By eliminating calcium, it can facilitate growth of cells in suspension cultures. AL221A is alpha modification of Minimum Essential Medium Eagle with L-glutamine, sodium bicarbonate, deoxyribonucleosides and ribonucleosides. This medium does not contain ascorbic acid.

[AL223A] RPMI 1640 medium is extensively used for a wide range of anchorage dependant cell lines. The medium is known to support growth of cells both in presence of 5-20% FBS and in absence of FBS. AL223A contains 2mM L-glutamine, 10mM HEPES buffer, 4.5gms per litre glucose, 2.0gms per litre sodium bicarbonate and does not contain sodium pyruvate.

[AL223S] RPMI 1640 medium is extensively used for a wide range of anchorage dependant cell lines. The medium is known to support growth of cells both in presence of 5-20% FBS and in absence of FBS. AL223S contains 2mM L-glutamine, 10mM HEPES Buffer, 4.5gms per litre glucose, 1.5gms per litre sodium bicarbonate. It does not contain sodium pyruvate.

[AL226] CMRL media are based on the composition of Medium 199. CMRL 1066 Medium with sodium bicarbonate. It does not contain L-Glutamine and Phenol red.

[AL230A] Iscove's Modified Dulbecco's Medium(IMDM) is an enriched modification of Dulbecco's Modified Eagle's Medium wherein serum can be partially or totally replaced by chemically defined substances. The medium when appropriately supplemented supports good growth of precursor cells of erythrocytes and macrophages. The medium also supports good growth of T and B lymphocytes and a variety of hybrid cells under serum free or reduced serum conditions. AL230A contains L-glutamine, 25mM HEPES buffer and sodium bicarbonate. It does not contain glucose and sodium bicarbonate.

[AL240] William's Medium E was developed by Williams and Gunn for the isolation and long term maintenance of adult rat liver epithelial cells. AL240 is William's Medium with sodium bicarbonate. It does not contain L-glutamine and phenol red.
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