[CCK031] The EZcountTM BrdU Cell Assay kit is designed for determination of cell proliferation and / or effect ofcytotoxic agent. When cells are cultured with labeling medium containing Pyrimidine analogue 5-bromo-2’-deoxyuridine(BrdU), BrdU gets incorporated in the DNA of proliferating cells in place of thymidine. BrdU labelled proliferating cells can be detected using Anti-BrdU antibody. The labeling medium is removed, and cells are fixed and DNA is denatured using fixation/denaturation solution to make BrdU accessible to the Anti-BrdU-HRP antibody. HRP substrate TMB is added for color development. The magnitude of the absorbance for the developed color directly correlates to the quantity of BrdU incorporated into cells, which is a direct indication of cell proliferation.

[CCK051] The EZcountTM ATP Cell Assay Kit is designed for determination of cell viability and cell proliferation and/or effect of cytotoxic agents. The kit is based on the generation of luminescence as ATP catalyzes the reaction between D-luciferin and enzyme luciferase. ATP is ubiquitously present in all viable, metabolically active cells. On the other hand, cell death is characterized by a significant decrease in the ATP level.

[CCK053] The EZcountTM MTS Cell Assay kit is designed for determination of cell viability and cell proliferation and/or effect of cytotoxic agent. This kit is based on the quantitative measurement of extracellular reduction of the water soluble tetrazolium dye MTS [2-(4-Iodophenyl)-3-4-nitrophenyl)-5-(2,4-disulfophenyl)-2H-tetrazolium sodium salt] to water soluble coloured formazan product by metabolically active cells.

[CCK037] L-glutamate is most abundant intracellular amino acid whereas L-glutamine is the most abundant extracellular amino acid. Estimation of L-glutamate and L-glutamine levels in the culture medium is thus indicative of the cellular metabolism and health of the cells. CCK037 is designed for estimation of concentration of these amino acids in cell culture samples such as cell lysates and spent media.

[CCK015] The EZcountTM XTT Cell Assay kit is designed for determination of cell viability and cell proliferation and / or effect of cytotoxic agent. This kit is based on quantitative measurement of extracellular reduction of XTT to water soluble orange coloured formazan derivative by metabolically active cells.

[TC724] Fluo-4 AM

Net weight: 0,0005 kg

xxx DRY ICE (-20 C°) xxx

[CCK035] Alkaline phosphatase(ALP) is a group of isoenzymes that hydrolyze phosphate esters and generate inorganic phosphate at an alkaline pH. CCK035 is a simple colorimetric kit designed to detect the presenceof alkaline phosphatase activity.

[CCK058] CCK058 has been designed for rapid determination of cell cytotoxicity in a one step reaction. Non-toxicity of the LDH reagent allows performing the assay in a single culture vessel i.e. in homogenous format. The reagent can accurately determine cell cytotoxicity in the samples containing mixed population of viable and dead cells. This feature makes the method suitable for high throughput automation wherein minimum handling and manipulations are required.

[CCK079] This kit is based on the quantitative measurement of potential dependent presence of JC-1 dye accumulated in mitochondria. Consequently, mitochondrial depolarization is indicated by an increase in the green/red fluorescence intensity ratio. The potential-sensitive color shift is due to concentration-dependent formation of red fluorescent J aggregates.

[CCK052] The EZcount™ Sulphorhodamine B cell assay kit is designed for determination of cell viability and cell proliferation and/or effect of cytotoxic agents. This kit is based on the quantitative measurement of cellular protein.

[CCK063] EZdetectTM Cell Senescence Detection Kit is designed for detection of cell senescence in culture. This kit is based on the qualitative detection of senescent cells that exhibit increased activity of acidic β-galactosidase, termed senescence-associated β-galactosidase(SA-β-gal). SA-β-gal is the most widely accepted marker of senescent cells.

[CCK061] It is based on the reduction of nitrate(NO3-) to nitrite(NO2-) by a reducing agent at 37°C. Converted nitrite and endogenous nitrite are collectively converted by Griess reagent to a blue colored azo compound. This compound can be measured spectrophotometrically between 580-630nm and absorbance is directly proportional to the total nitric oxide concentration in the sample which is calculated from standard plot of NaNO3.

[CCK036] Lactate dehydrogenase(LDH) is a cytoplasmic enzyme present in all mammalian cells. Plasma membrane of live cells is impermeable to LDH. However it is rapidly released into the culture medium when plasma membrane is damaged. CCK036 has been designed for rapid determination of cell cytotoxicity using an enzyme coupled two step reaction.

[CCK009] Mycoplasmas are the smallest known, simple prokaryotes, constituting one of the most widely spread contaminants found in cell culture. This kit has been designed for detection of mycoplasma based on amplification of spacer region between 16S and 23S rRNA genomic DNA sequence. This sequence is highly conserved between various mycoplasma species. This allows for detection of commonly encountered cell culture contaminants. Eukaryotic DNA is not amplified. A successful PCR reaction is indicated by a distinct band of 350-400bp derived from the internal control DNA on the agarose gel.

[CCK022] Mycoplasmas are the smallest known, simple prokaryotes, constituting one of the most widely spread contaminants found in cell culture. PCR based detection of mycoplasma is one of the most convenient method. This kit has been designed for detection of mycoplasma based on amplification of 16S rRNA genomic DNA sequence. This sequence is highly conserved between various mycoplasma species. Eukaryotic DNA is not amplified. A successful PCR reaction is indicated by a distinct band of 502 - 520bp derived from the internal control DNA on the agarose gel.

[CCK069] Glucos-6-Phosphate Dehydrogenase(G6PD) is a cytosolic enzyme present in all cells including RBCs. Plasma membrane of live cells is impermeable to G6PD. However, it is rapidly released into the culture medium when plasma membrane is damaged. The quantity of the released G6PD is used as a measure of cell cytotoxicity. CCK069 has been designed for rapid determination of cell death. The dye used in the assay is non-radioactive, water soluble and stable in most of the culture media.

[CCK023] Melondialdehyde(MDA) is one of such TBARS used for estimation of lipid peroxidation. This assay is based on the reaction of MDA with a chromogenic agent thiobarbituric acid(TBA) at high temperature and acidic conditions to form MDA-TBA adduct(1:2). This complex has absorption maximum at 532nm and can be measured spectrophotometrically between 530 - 540nm. Absorbance is directly proportional to the TBARS concentration in the sample.

[CCK006] EZkillTM Mycoplasma Elimination Kit is an antibiotic based elimination kit designed to eliminate mycoplasma from cell culture. Mycoplasma contamination of cell culture is a major problem in both research and industrial production. Ideally it is recommended to discard the contaminated culture after autoclaving. Only in cases where cell line is very important and non-replaceable, treatment for elimination should be considered. Antibiotic based method is the most common and effective method with no marked cytotoxicity.

[CCK078] EZAssayTM ROS Kit is designed for determination of reactive oxygen species activity within a cell using a cell permeable fluorogenic dye. Once fluorogenic dye diffuses inside the cells it gets deacetylated to non-fluorescent compound due to esterases. In presence of ROS, non fluorescent dye immediately gets oxidized. Fluorogenic dye is easily detectable using wavelength of excitation-488nm and emission-528nm. The fluorescent intensity produced is directly proportional to ROS level in the cells.

[CCK072] This assay kit has been designed for measuring the ferric reducing ability of test sample. In this assay, ferric ion-chromogen complex gets reduced to colored ferrous ion at low pH. FRAP values are obtained by comparing the absorbance change at 593nm in test reaction mixtures with those containing ferrous ions in known concentration.