[TCL190] HEPES is a zwitterionic organic buffering agent. It is stable, water soluble and does not react with divalent cations. It has a pKa of 7.5 at 37°C and offers an optimal buffering range of pH 6.8 - 8.2. It maintains the physiological pH despite changes in the carbon dioxide concentrations which take place due to cellular metabolism. Unlike the bicarbonate buffers which require CO2, HEPES containing media do not need CO2 atmosphere for buffering. HEPES is generally used at a concentration of 10mM to 25mM for cell culture application. TCL190 is 1M HEPES solution prepared in sterile filtered saline solution.

Kühlware (2°C - 8°C)

[TL1002] Earle's balanced salt solution is designed to equilibrate with a 5% CO2 in air mixture. Earle's balanced salt solution with phenol red and sodium bicarbonate hence requires the cells to be grown in a 5% CO2 environment.

Net weight: 0,5 kg

[TL1003] Hanks' balanced salt solution with phenol red and sodium bicarbonate. It is designed for use with cells maintained in less CO2 environment or CO2 free environment.

Net weight: TL1003-500ML: 0,5 kg
TL1003-5x100ML: 0,5 kg, price for this Variant on request

[TL1006] 1X Dulbecco's Phosphate Buffered Saline is without phenol red, calcium and magnesium. It is most commonly used for tissue disaggregation and monolayer dispersal since presence of calcium and magnesium ions may hinder the trypsin activity.

Net weight: TL1006-1000ML: 1 kg
TL1006-500ML: 0,5 kg
TL1006-5x100ML: 0,5 kg, price for this Variant on request

[TL1010] Hanks' balanced salt solution with sodium bicarbonate. It is designed for use with cells maintained in less CO2 environment or CO2 free environment. It does not contain phenol red.

Net weight: 0,5 kg

[TL1011] Spinner balanced salts, Eagle is with phenol red and sodium bicarbonate. It does not contain calcium chloride.

Net weight: 0,5 kg

[TL1019] Hanks' balanced salt solution is with phenol red. It is designed for use with cells maintained in less CO2 environment or CO2 free environment. It does not contain sodium bicarbonate.

[TL1021] 10X Hanks' balanced salt solution is with phenol red. It does not contain sodium bicarbonate. It is designed for use with cells maintained in less CO2 environment or CO2 free environment.

Net weight: 0,5 kg

[TL1022] 10X Dulbecco's Phosphate Buffered Saline is without calcium, magnesium and Phenol red. It is most commonly used for tissue disaggregation and monolayer dispersal since presence of calcium and magnesium ions may hinder the trypsin activity.

Net weight: 0,5 kg

[TL1023] Dulbecco's Phosphate Buffered Saline with calcium and magnesium. It is most commonly used for tissue disaggregation and monolayer dispersal since presence of calcium and magnesium ions may hinder the trypsin activity.

[TL1024] Hanks' balanced salt solution with phenol red and sodium bicarbonate. It is designed for use with cells maintained in less CO2 environment or CO2 free environment. It does not contain calcium.

Net weight: 0,5 kg

[TL1031] 1X Phosphate Buffered Saline, pH 7.2 does not contain calcium and magnesium, hence most commonly used for tissue disaggregation and monolayer dispersal since presence of calcium and magnesium ions may hinder the trypsin activity.

[TL1032] 10X Phosphate Buffered Saline, pH 7.2 does not contain calcium and magnesium hence most commonly used for tissue disaggregation and monolayer dispersal since presence of calcium and magnesium ions may hinder the trypsin activity.

Net weight: 0,5 kg

[TL1033] 20X Phosphate Buffered Saline, pH 7.2 does not contain calcium and magnesium and hencecommonly used for tissue disaggregation and monolayer dispersal since presence of calcium and magnesium ions may hinder the trypsin activity.

Net weight: 0,5 kg

[TL1097] Krebs-Ringer Bicarbonate Buffer is with 1.8gms Glucose per liter and sodium bicarbonate.

Net weight: 0,5 kg

[TL1098] Hanks' balanced salt solution is designed to equilibrate with air, hence does not require CO2 air mixture. This balanced salt solution with sodium bicarbonate and is designed for use with cells maintained in less CO2 environment or CO2 free environment. It does not contain calcium, magnesium and phenol red.

Net weight: 0,5 kg

[TL1099] 10X Phosphate Buffered Saline, pH 7.4 does not contain calcium and magnesium hence most commonly used for tissue disaggregation and monolayer dispersal since presence of calcium and magnesium ions may hinder the trypsin activity.

Net weight: 0,5 kg

[TL1101] 1X Phosphate Buffered Saline, pH 7.4 does not contain calcium and magnesium, hence most commonly used for tissue disaggregation and monolayer dispersal since presence of calcium and magnesium ions may hinder the trypsin activity.

Net weight: 0,5 kg

[TL1104] 1X Sodium Phosphate Buffered Saline. It is also used for routine immunohistochemical testing and as a general purpose solution for washing cells in various hematological and molecular biology procedures.

Net weight: 0,5 kg

[TL1106] Dulbecco's Phosphate Buffered Saline without Phenol red, contains 1gm glucose per litre, 36mg sodium pyruvate per litre, calcium and magnesium.

Net weight: 0,5 kg