Lab-in-a-cell SPAchip®

Advanced technology in living single cell

Introducing SPAchip® technology – a revolutionary solution for monitoring cellular pathways in real-time! Our intracellular silicon microchips are designed to be easily internalized in living cells without inducing physiological changes or affecting cell viability

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"Thanks to a cutting-edge miniaturization process, the SPAchips offer a highly precise and accurate way to analyze intracellular processes with multiple fluorescent probes. By simply changing the SPAchip® fluorescence, you can gain valuable insights into cellular activity. With the SPAchip® kit, you can save time and costs by using it with high-content screening platforms and imaging analyzers. It offers a user-friendly, yet powerful solution for monitoring cellular pathways that will help you take your research to the next level"

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"What are the key features of CASY?"


  • Highest reproducibility and accuracy for anything you want to analyze
  • No sample preparation, easy use
  • High speed at low cost
  • Transparent results and quality control
  • Thousands of publications and thousands of installations
  • GMP/GLP compliant
  • Optional 21CFR Part 11 module

CASY Cell Counter & Analyzer – always the best choice, whatever you want to analyze. From large stem cell aggregates to small yeast or even bacteria. CASY provides unmatched precision and reproducibility.

CASY can do it all – the unique detection mechanism will support you even with challenging applications

"How can SPAchip® technology enhance your research on cellular pathways?"

#SPAchip® technology

The SPAchip® is the first intracellular device that can be multiplexed with probes emitting in the same wavelength. Surface functionalization techniques enable SPAchip® to be printed with nucleic acids, proteins, carbohydrates, lipids, or synthetic chemicals for tracking cell metabolism.  

Transforming Cellular Analysis: The Power of SPAchip®

Imagine a cell-based assay so advanced it can work inside a living single cell, offering real-time analysis and ongoing insights. SPAchip® technology turns this revolutionary idea into reality, opening new doors in the study of cellular metabolism and disease.

Introducing SPAchip® Technology

SPAchip® is where cellular biology and microelectronics merge, introducing an innovative Suspended-Planar Array chip technology that sheds unprecedented studies on cellular processes in a way never seen before or affecting cell viability. 

SPAchips are intracellular silicon microchips functionalized with several biomolecules ( uorescence probes, proteins, peptides, and other) designed to  lm any cellular pathway by monitoring it in real time without inducing physiological changes

In this  lm, SPAchip® offers a dynamic description about cell sensing from inside at real-time. 

mechanisms that were previously unavailable. As a result of this advancement, we are able to study living cells more closely, providing deeper insights into cellular behavior and

The SPAchip’s capabilities allow researchers to capture even subtle changes in biological processes with high sensitivity and accuracy.

The SPAchip® Difference: A Leap in Live Cell Analysis

  • Multiplex Capability: SPAchip® is the first intracellular device capable of being multiplexed with probes emitting in the same wavelength, revolutionizing how we study cellular metabolism.
  • Surface Functionalization: this technology enables the printing of SPAchips with a wide array of biomolecules, facilitating comprehensive tracking of cellular activities and metabolism.
  • Non-Invasive: SPAchips are non-toxic, ensuring long-term intracellular measurements can be taken without harming the cell.
  • Enhanced Sensitivity: by concentrating the fluorescent probe on the chip’s surface rather than in solution, SPAchip® technology offers unparalleled sensitivity in detecting cellular changes.
  • Eco-Friendly: utilizes fewer plastics and consumables, aligning with sustainable lab practices.
  • Versatile Application: SPAchip´s workflow is designed to complement existing lab infrastructures, integrating seamlessly with a variety of platforms for a wide range of applications.


"How SPAchip® works in combination with the current laboratory equipments?"


Enhancing Workflow Efficiency with SPAchip® Technology

Below, we present a comprehensive workflow for the analysis of cell-based imaging assays utilizing high-content imaging and cellular analysis software. Our user-friendly assay kits cater to the needs of life science research, drug discovery, and bioassays, providing a seamless experience. These kits are meticulously designed to seamlessly integrate with the latest Cell Imaging instruments, ensuring optimal performance and reliable results.

Discover SPAchip® Today  

SPAchip® Detection kits.

SPAchip® technology is the future of live cell imaging and drug discovery. You can start your experience with our

Discover SPAchip® Today

Take your research to a new level with CytoCHECK SPAchip® Detection kits. >> Learn More

Revolutionizing Drug Discovery 

SPAchip® is not just an innovation in live cell imaging; it’s a transformative tool for drug discovery.

By enabling detailed analysis  of single-cell responses to drug candidates, SPAchip® technology accelerates the identi cation of new targets and therapeutic 

opportunities, promising a new era of personalized medicine and ef cient drug development.

  • Target-Based Innovation: A4cell’s technology enhances target-based drug discovery by facilitating the design and printing of custom chemical compounds. This capability allows for the quick and direct detection of specific targets, streamlining the drug development process. 
  • Phenotypic Discovery Leap: It advances phenotypic drug discovery by enabling the printing and analysis of various molecules. This breakthrough assists in identifying new therapeutic targets, mechanisms of action, and potential drug candidates, marking a significant stride forward. 
  • Optimizing HCS Platforms: A4cell optimizes High Content Screening (HCS) platforms, improving the e iciency of identifying lead compounds, assessing drug e icacy, and characterizing cellular phenotypes.

The synergy between live cell imaging and SPAchip® technology fosters the creation of innovative assays and experimental protocols, broadening the horizons of biological research. Additionally, this approach minimizes phototoxicity and uses only one  uorescence channel, eliminating the need for extra experiments and saving up to 60% on time and costs. The A4cell Multiplex Detection kit unveils new insights, uncovering patterns and correlations previously hidden, enriching our understanding in drug discovery and steering us  towards personalized medicine.

Applications and Cell Types

Because each cell is a Storyteller….

The SPAchip® abilities to monitor living single cells during drug discovery offers a deeper understanding of drug responses. Leads to the identi cation of new targets and therapeutic opportunities, and can improve the ef ciency and safety of the drug development process. It is a powerful tool that has the potential to accelerate drug discovery and improve patient outcomes.

Timelapse of HEK293 cell line (from Human Embryonic Kidney) spheroid formation with internalization of CytoCHECK SPAchip® pH Single-Detection Kit Green. DRAQ5™ in red stained live nuclei. SPAchip® technology allows pH measurement at the different time points of spheroid formation. HEK293 cells were incubated with CytoCHECK SPAchip® pH Single-Detection to allow spheroid formation at 24 A), 28 B), 48 C), and 52 hours D).

A4cell is delighted to present you a disruptive technology able to analyze living single cells for extended periods offering innovative

Cell-based assays with huge advantages:




Connect us to gain from our SPAchip®technology!

#SPAchip® #Application


  • Cell proliferation
  • Apoptosis
  • Cell migration
  • Tumor Microenvironment
  • Inmuno oncology
  • Ferroptosis
  • Inflammation


  • Oxidative
  • Mitochondrial function
  • Acidification
  • Calcium signaling
  • Glucose consumption
  • Response to hypoxia
  • Autophagy


  • Cell culture quality control
  • Differentation processesz


  • Mitochondrial function
  • Acidification
  • Calcium signaling
  • Glucose consumption
  • Response to hypoxia
  • Autophagia


  • Cell culture quality control
  • Viability and proliferation
  • Cell functional properties

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