[TCL015] Pyruvate is an intermediary organic acid metabolite in glycolysis and the EMP pathway. It initiates the Kreb's cycle and is thus involved in the production of ATP within the cell. Addition of Sodium pyruvate to tissue culture medium provides both an energy source and a carbon skeleton for anabolic processes. It helps in maintaining certain specialized cells and necessary when the serum concentration is reduced in the medium. TCL015 is a 100mM solution of sodium pyruvate prepared in cell culture grade water. It is commonly used at a concentration of 1mM in cell culture applications.

Net weight: 0,1 kg

refrigerated goods (2°C - 8°C)

[TCL016] TCL016 is molecular biology grade sterile filtered water, treated with 0.1% Diethylpyrocarbonate(DEPC). It is prepared by passing water through continuous process which includes water pretreatment, reverse osmosis, deionisation, and filtration. It is produced specifically for molecular biology applications like DNA/RNA purification, protein applications and experiments involving RNA. DEPC inactivates RNases by carboxylation of specific amino acid side chains in the protein.

Net weight: TCL016-10x500ML: 5 kg
TCL016-20x100ML: 2 kg
TCL016-500ML: 0,5 kg

[TCL018] TCL018 is molecular biology grade sterile filtered water, treated with 0.1% diethylpyrocarbonate(DEPC). It is prepared by passing water through continuous process which includes water pretreatment, reverse osmosis, deionization, distillation and filtration. It is produced specifically for molecular biology applications like DNA/ RNA purification, protein applications and experiments involving RNA.

Net weight: TCL018-10x500ML: 5 kg
TCL018-20x100ML: 2 kg
TCL018-500ML: 0,5 kg

[TCL019] TCL019 is tissue culture grade sterile filtered water. It is prepared by passing water through continuous process which includes water pretreatment, reverse osmosis, deionisation, and filtration. It is produced specifically for cell culture applications like reconstitution of powdered media and other cell culture reagents.

Net weigth: TCL019-10x500ML: 5 kg
TCL019-20x100ML: 2 kg
TCL019-500ML: 0,5 kg

[TCL020] Versene (EDTA) solution is sterile filtered solution formulated to contain 0.1% EDTA in Dulbecco’s phosphate buffered saline.

Net weight: TCL020-2x500ML: 1 kg
TCL020-5x100ML: 0,5 kg

[TCL021] HEPES is a zwitterionic organic buffering agent. It is stable, water soluble and does not react with divalent cations. It has a pKa of 7.5 at 37°C and offers an optimal buffering range of pH 6.8 - 8.2. It maintains the physiological pH despite changes in the carbon dioxide concentrations which take place due to cellular metabolism. Unlike the bicarbonate buffers which require CO2, HEPES containing media do not need CO2 atmosphere for buffering. HEPES is generally used at a concentration of 10mM to 25mM for cell culture application. TCL021 is 1M HEPES solution prepared in sterile tissue culture grade water.

Net weigth: TCL021-2X500ML: 1 kg
TCL021-500ML: 0,5 kg
TCL021-5X50ML: 0,25 kg
TCL021-100ML: 0,1 kg

refrigerated goods (2°C - 8°C)

[TCL022] This product contains 0.25% Trypsin, 0.5% PVP, 0.02% EDTA and 0.05% Glucose in Dulbecco’s phosphate buffered saline.

Net weight: TCL022-500ML: 0,5 kg
TCL022-5X100ML: 0,5 kg
TCL022-100ML: 0,1 kg

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[TCL024] Potassium bicarbonate is used to supplement Sheilds and Sangs M3 Insect Medium(IM004). TCL024 is a sterile filtered solution formulated to contain 7.5% potassium bicarbonate.

Net weight: 0,5 kg

[TCL028] ZymeFree™ works as quick as trypsin on most cell lines. It holds the advantage over enzyme based dissociation solutions as it does not cause cytotoxicity, even after cells are exposed to it for prolonged period.

Net weight: TCL028-2X500ML: 1 kg
TCL028-100ML: 0,1 kg

refrigerated goods (2°C - 8°C)

[TCL030] L-glutamine is an essential amino acid required by all mammalian and insect cells grown in culture. L-glutamine degrades rapidly in solution through an intramolecular cyclization reaction forming ammonia as a byproduct. HiGlutaXL™ supplement contains the stabilised dipeptide form of L-glutamine, L-alanyl-L-glutamine. The thermostable nature of L-alanyl-L glutamine obviates the need of addition of L-glutamine at the time of use and allows liquid media to be stored at 4°C for longer periods. TCL030 is a sterile filtered 200mM solution of L-alanyl-L-glutamine prepared in 0.85% normal saline.

Net weight: 0,1 kg

refrigerated goods (2°C - 8°C)

[TCL031] Trypsin is a serine protease derived from porcine pancreas. Ethylenediaminetetraacetic acid (EDTA), a chelating agent is often added to enhance enzymatic activity of trypsin solution. This product contains 0.1% Trypsin, 0.02% EDTA, 0.05% Glucose and Phenol red in Dulbecco’s phosphate buffered saline.

Net weight: TCL031-500ML: 0,5 kg
TCL031-5X100ML: 0,5 kg
TCL031-100ML: 0,1 kg

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[TCL033] Trypsin is a serine protease derived from porcine pancreas. Ethylenediaminetetraacetic acid (EDTA), a chelating agent is often added to enhance enzymatic activity of trypsin solution. This product contains 0.05% Trypsin and 0.02% EDTA and phenol red in Hank’s balanced salt solution.

Net weight: TCL033-500ML: 0,5 kg
TCL033-5X100ML: 0,5 kg
TCL033-100ML: 0,1 kg

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[TCL034] Trypsin is a serine protease derived from porcine pancreas. Ethylenediaminetetraacetic acid (EDTA), a chelating agent is often added to enhance enzymatic activity of trypsin solution. This product contains 0.5% Trypsin and 0.2% EDTA in 0.85% normal saline. It does not contain Phenol red.

Net weight: TCL034-500ML: 0,5 kg
TCL034-100ML: 0,1 kg

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[TCL041] Yeast Extract is the water-soluble portion of autolyzed yeast. It provides naturally occurring B-complex vitamins, nitrogen, amino acids and carbon in cell culture media. TCL041 is a sterile filtered solution of Yeast Extract. It is most commonly used as an enrichment supplement to grow Mycoplasma.

Net weight: TCL041-500ML: 0,5 kg
TCL041-100ML: 0,1 kg

[TCL042] Trypsin is a serine protease derived from porcine pancreas. Ethylenediaminetetraacetic acid (EDTA), a chelating agent is often added to enhance enzymatic activity of trypsin solution. This product contains 0.25% Trypsin and 0.1% EDTA in Hank’s balanced salt solution. It does not contain Phenol red.

Net weight: TCL042-500ML: 0,5 kg
TCL042-5X100ML: 0,5 kg
TCL042-100ML: 0,1 kg

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[TCL047] Trypsin is a serine protease derived from porcine pancreas. This product is 0.25% Trypsin in Hank’s balanced salt solution.

Net weight:TCL047-500ML: 0,5 kg
TCL047-5X100ML: 0,5 kg
TCL047-100ML: 0,1 kg

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[TCL048] Trypsin-EDTA solution is is 0.25% Trypsin, 0.038% EDTA in Hank’s balanced salt solution and phenol red. It does not contain calcium and magnesium.

Net weigth: TCL048-500ML: 0,5 kg
TCL048-5X100ML: 0,5 kg
TCL048-100ML: 0,1 kg

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[TCL049] Trypsin-EDTA solution contains 0.25% Trypsin and 0.02% EDTA in Hank’s balanced salt solution.

Net weight: TCL049-500ML: 0,5 kg
TCL049-5X100ML: 0,5 kg
TCL049-100ML: 0,1 kg

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[TCL059] Gelatin is a protein produced by partial hydrolysis of collagen extracted from boiled bones, hides and skin, connective tissues, organs and some intestines of domesticated animals like cattle, pigs and horses. Gelatin is widely used in cell culture for coating the cell culture plates and flasks to improve the cell attachment for certain types of cells. TCL059 is a sterile filtered 2% solution of Gelatin from bovine skin prepared in tissue culture grade water. Gelatin is generally used in cell culture applications at a concentration of 0.1%.

Net weight: 0,1 kg

[TCL068] Trypsin Inhibitor is soyabean trypsin inhibitor solution formulated to contain 1mg per ml trypsin inhibitor in Dulbecco's Phosphate Buffered Saline.

Net weight: 0,1 kg

refrigerated goods (2°C - 8°C)