EZcountTM BrdU Cell Assay Kit
[CCK031] The EZcountTM BrdU Cell Assay kit is designed for determination of cell proliferation and / or effect ofcytotoxic agent. When cells are cultured with labeling medium containing Pyrimidine analogue 5-bromo-2’-deoxyuridine(BrdU), BrdU gets incorporated in the DNA of proliferating cells in place of thymidine. BrdU labelled proliferating cells can be detected using Anti-BrdU antibody. The labeling medium is removed, and cells are fixed and DNA is denatured using fixation/denaturation solution to make BrdU accessible to the Anti-BrdU-HRP antibody. HRP substrate TMB is added for color development. The magnitude of the absorbance for the developed color directly correlates to the quantity of BrdU incorporated into cells, which is a direct indication of cell proliferation.
[CCK031] The EZcountTM BrdU Cell Assay kit is designed for determination of cell proliferation and / or effect ofcytotoxic agent. When cells are cultured with labeling medium containing Pyrimidine analogue 5-bromo-2’-deoxyuridine(BrdU), BrdU gets incorporated in the DNA of proliferating cells in place of thymidine. BrdU labelled proliferating cells can be detected using Anti-BrdU antibody. The labeling medium is removed, and cells are fixed and DNA is denatured using fixation/denaturation solution to make BrdU accessible to the Anti-BrdU-HRP antibody. HRP substrate TMB is added for color development. The magnitude of the absorbance for the developed color directly correlates to the quantity of BrdU incorporated into cells, which is a direct indication of cell proliferation.
Specifications
| Units | 1000T |