[AL007A] DMEM is a modification of Basal Medium Eagle (BME) that contains four fold concentration of amino acids and vitamins. Dulbecco's Modified Eagle Medium contains 4.5gms glucose per litre, L-glutamine, 3.7 gms per litre sodium bicarbonate and sodium pyruvate.

[AL215A] Dulbecco’s Modified Eagle Medium / Nutrient Mixture F12 Ham (DMEM/F12, 1:1 mixture) was originally formulated for rat neuroblastoma cells and MDCK cells.AL215A is DMEM/Nutrient Mixture F-12 Ham with L-glutamine, sodium bicarbonate and 15mM HEPES buffer. It does not contain phenol red and trace elements elements.

[AL106A] Kaighn’s modification of Ham’s F-12 is a complex formulation of F-12 with increased amounts of amino acids and puruvate. Salts used in this formulation are as given by Konisberg. This modification supports growth and differentiation of rat and chicken cells and primary human liver cells. AL106A contains L-glutamine and sodium bicarbonate.

[AL025] Nutrient Mixture F-12 Ham is the medium of choice for the growth of cells of rodent origin, cloning of myeloma and hybridoma cells and performing clonal toxicity assay using CHO cells. AL025 contains sodium bicarbonate and does not contain L-glutamine.

[AL024] Ham's Nutrient Mixture F10 was designed for clonal growth of CHO cells and chick embryo cells under serum free conditions. It is now widely used for culturing a variety of cells including human diploid cells and white blood cells for chromosomal analysis and primary explants of rat, rabbit and chicken tissues. AL024 contains sodium bicarbonate and does not contain L-glutamine.

[AL138] NCTC 109 is one of the chemically defined medium; originally formulated to establish and maintain a strain of mouse cells, L929. The medium supports growth of fibroblast-like and epithelial- like cells of both normal and malignant origin from mice, hamsters, monkeys and humans. AL138 contains sodium bicarbonate and co-enzyme A. It does not contain L-glutamine and HEPES.

[AL046A] Minimum Essential Medium(MEM) was developed by Harry Eagle to meet the specific nutritional requirements of certain subtypes of HeLa cells and normal mammalian fibroblasts. By eliminating calcium, it can facilitate growth of cells in suspension cultures. AL046A contains Earle’s salts, L-glutamine, sodium bicarbonate and non-essential amino acids. It does not contain phenol red.

[AL006] DMEM is a modification of Basal Medium Eagle (BME) that contains four-fold concentration of amino acids and vitamins. Dulbecco's Modified Eagle Medium is with 1gm per litre glucose, sodium bicarbonate and sodium pyruvate. It does not contain L-glutamine.

[AL081] Minimum Essential Medium(MEM) was developed by Harry Eagle to meet the specific nutritional requirements of certain subtypes of HeLa cells and normal mammalian fibroblasts. By eliminating calcium, it can facilitate growth of cells in suspension cultures. AL081 is alpha modification of Minimum Essential Medium Eagle with sodium bicarbonate and nonessential amino acids. It does not contain deoxyribonucleosides, ribonucleosides and L-glutamine.

[AL149A] Dulbecco's Modified Eagle Medium with L-glutamine, 1gm per litre of glucose, 25mM HEPES buffer, sodium bicarbonate and sodium pyruvate. HEPES, a zwitterionic buffer having a pKa of 7.3 at 37ºC prevents the initial rise in pH that tends to occur at the initiation of a culture and increases the buffering capacity of the medium.

[AL140] Dulbecco’s Modified Eagle Medium / Nutrient Mixture F12 Ham (DMEM/F12, 1:1 mixture) was originally formulated for rat neuroblastoma cells and MDCK cells.AL140 is DMEM/Nutrient Mixture F-12 Ham with 15mM HEPES buffer and sodium bicarbonate. It does not contain L-glutamine and trace elements.

[AL067A] DMEM is a modification of Basal Medium Eagle (BME) that contains four fold concentration of amino acids and vitamins. DMEM with L-glutamine, 4.5gms per litre glucose, 25mM HEPES buffer and sodium bicarbonate. It does not contain sodium pyruvate.

[AL070S] Iscove's Modified Dulbecco's Medium(IMDM) is an enriched modification of Dulbecco's Modified Eagle's Medium wherein serum can be partially or totally replaced by chemically defined substances. The medium when appropriately supplemented supports good growth of precursor cells of erythrocytes and macrophages. The medium also supports good growth of T and B lymphocytes and a variety of hybrid cells under serum free or reduced serum conditions. AL070S contains 4mM L-glutamine, 4.5gms glucose per litre, 25mMHEPES buffer and 1.5 gms per litre sodium bicarbonate.

[AL070H] Iscove's Modified Dulbecco's Medium(IMDM) is an enriched modification of Dulbecco's Modified Eagle's Medium wherein serum can be partially or totally replaced by chemically defined substances. The medium when appropriately supplemented supports good growth of precursor cells of erythrocytes and macrophages. The medium also supports good growth of T and B lymphocytes and a variety of hybrid cells under serum free or reduced serum conditions. AL070H is hybridoma tested medium with L-glutamine, 25mM HEPES buffer and sodium bicarbonate.

[AL070A] Iscove's Modified Dulbecco's Medium(IMDM) is an enriched modification of Dulbecco's Modified Eagle's Medium wherein serum can be partially or totally replaced by chemically defined substances. The medium when appropriately supplemented supports good growth of precursor cells of erythrocytes and macrophages. The medium also supports good growth of T and B lymphocytes and a variety of hybrid cells under serum free or reduced serum conditions. AL070A contains L-glutamine, 25mM HEPES buffer and 3.024 gms per litre sodium bicarbonate.

[AL070] Iscove's Modified Dulbecco's Medium(IMDM) is an enriched modification of Dulbecco's Modified Eagle's Medium wherein serum can be partially or totally replaced by chemically defined substances. The medium when appropriately supplemented supports good growth of precursor cells of erythrocytes and macrophages. The medium also supports good growth of T and B lymphocytes and a variety of hybrid cells under serum free or reduced serum conditions. AL070 contains 25mM HEPES buffer and sodium bicarbonate. It does not contain L-glutamine.

[AL020A] Minimum Essential Medium(MEM) was developed by Harry Eagle to meet the specific nutritional requirements of certain subtypes of HeLa cells and normal mammalian fibroblasts. By eliminating calcium, it can facilitate growth of cells in suspension cultures. AL020A contains Earle's salts, L-glutamine and 2.2gms per litre sodium bicarbonate. It does not contain non-essential amino acids.

[AL019] Minimum Essential Medium(MEM) was developed by Harry Eagle to meet the specific nutritional requirements of certain subtypes of HeLa cells and normal mammalian fibroblasts. By eliminating calcium, it can facilitate growth of cells in suspension cultures. AL019 is Minimum Essential Medium with Spinner’s balanced salts for suspension culture. It does not contain L-glutamine.

[AL014A] Medium 199 was the first nutritionally defined medium developed by Morgan, Morton, and Parker in 1950 for nutritional studies on primary chick embryo fibroblasts in the absence of any additives. Media 199 are presently used for the maintenance of non-transformed cells, vaccine and virus production and primary explants of epithelial cells. AL014A contains Earle's salts, L-glutamine and sodium bicarbonate.

[AL267] Medium 199 was the first nutritionally defined medium developed by Morgan, Morton, and Parker in 1950 for nutritional studies on primary chick embryo fibroblasts in the absence of any additives. Media 199 are presently used for the maintenance of non-transformed cells, vaccine and virus production and primary explants of epithelial cells. AL267 contains Hank's salts and sodium bicarbonate. It does not contain L-glutamine.