Cell Analysis ELISA Kits
Made with HumanKien human cell-expressed proteins
Due to the highest quality immunogens in the industry, the capture and detection antibodies have the highest affinity for human proteins.
To best mimic the human sample analyte for optimizing the ELISA parameters, HumanKine® proteins are used as the standard. AuthentiKine™ ELISA kits are well validated in natural samples like human serum and plasma
Cell Analysis ELISA Kits
"What are the key features of ELISA Kits?"
#elisa-kit-meets-all-demands
Proteintech ELISA kits – simple and elegant solution for your immunoassay needs
- Ultra-sensitive kits between 10X and 1000X more sensitive than kits sold by leading competitors
- The only kits made using actual human proteins from state-of-the-art HumanKine® human Cell-expressed cytokines and growth factors.
The procedure is straightforward and allows you to detect and quantify your target protein, reliably and accurately, in a range of biological samples in the space of a morning or afternoon. Proteintech’s ELISA kits are based on the two-site sandwich immunoassay principle, in which two highly specific, non-competitive antibodies are used in the detection of a target analyte: one for capture, and one for detection.
Briefly, steps involved in making Proteintech’s sandwich ELISA kits include:
- Careful selection of antibody pairs to ensure optimum signal
- Calibration with NIBSC/WHO Standards
- Testing with multiple sample types, e.g., sera, plasma, cell culture supernatant
- Testing of intra- and inter-assay precision
- Linearity testing
- Recovery testing
- Kit stability testing
ELISA sensitivity: AuthentiKine™ vs Competitors
Human APOL1 ELISA Kit
APOL1 is involved in the formation and efflux of cholesteryl esters and lipid transport. Proteintech offers a human-specific APOL1 ELISA kit, with high sensitivity and broad assay range.
The KE00047 kit is a quantitative measurement of human APOL1 protein in serum and plasma. It has recently been cited in top scientific publications as a useful tool regarding HIV, CKD, and frontotemporal dementia studies. ¹ ² ³
• Tests: 1 x 96 well plate, Sandwich ELISA for Serum, plasma
• Assay Time: 3.5 hours
• Sensitivity: 70 pg/mL
• Range: 156 – 10000 pg/mL
• Regulatory: Research use only
References
¹ Vascular smooth muscle cells contribute to APOL1-induced podocyte injury in HIV milieu, (PMID: 25796344).
² Novel diagnostic cerebrospinal fluid biomarkers for pathologic subtypes of frontotemporal dementia identified by proteomics, (PMID: 27239539).
³ A tripartite complex of suPAR, APOL1 risk variants and αvβ3 integrin on podocytes mediates chronic kidney disease, (PMID: 28650456).
Sensitivity pg./ml
ELISA KIT | Page | ELISA KIT | Page | ELISA KIT | Page |
CD31 | 6 | CEACAMS | 6 | PD-11 | 7 |
CD3E | 6 | PD1 | 7 |
Interleukin
