[TC115] EDTA free acid anhydrous

[CCK071] It is based on ability of antioxidants in the sample to reduce copper(II)-chromogen oxidant complex. Absorbance of the reduced copper(I)-chromogen complex is indicative of antioxidant capacity of the test sample.

[CCK078] EZAssayTM ROS Kit is designed for determination of reactive oxygen species activity within a cell using a cell permeable fluorogenic dye. Once fluorogenic dye diffuses inside the cells it gets deacetylated to non-fluorescent compound due to esterases. In presence of ROS, non fluorescent dye immediately gets oxidized. Fluorogenic dye is easily detectable using wavelength of excitation-488nm and emission-528nm. The fluorescent intensity produced is directly proportional to ROS level in the cells.

[CCK035] Alkaline phosphatase(ALP) is a group of isoenzymes that hydrolyze phosphate esters and generate inorganic phosphate at an alkaline pH. CCK035 is a simple colorimetric kit designed to detect the presenceof alkaline phosphatase activity.

[CCK072] This assay kit has been designed for measuring the ferric reducing ability of test sample. In this assay, ferric ion-chromogen complex gets reduced to colored ferrous ion at low pH. FRAP values are obtained by comparing the absorbance change at 593nm in test reaction mixtures with those containing ferrous ions in known concentration.

[CCK028] EZAssayTM GST Activity Estimation kit is simple and convenient tool for sensitive and reproducible detection and quantitation of GST activity in an array of samples like serum, cell lysates and tissue homogenates. This assay is based on the GST-catalyzed reaction between reduced glutathione and the GST substrate 1-chloro-2,4-dinitrobeneze(CDNB). CDNB can react with the broadest range of GST isoenzymes. The conjugation of glutathione to the CDNB substrate results in an increase in absorbance at 340nm.

[CCK037] L-glutamate is most abundant intracellular amino acid whereas L-glutamine is the most abundant extracellular amino acid. Estimation of L-glutamate and L-glutamine levels in the culture medium is thus indicative of the cellular metabolism and health of the cells. CCK037 is designed for estimation of concentration of these amino acids in cell culture samples such as cell lysates and spent media.

[CCK079] This kit is based on the quantitative measurement of potential dependent presence of JC-1 dye accumulated in mitochondria. Consequently, mitochondrial depolarization is indicated by an increase in the green/red fluorescence intensity ratio. The potential-sensitive color shift is due to concentration-dependent formation of red fluorescent J aggregates.

[CCK023] Melondialdehyde(MDA) is one of such TBARS used for estimation of lipid peroxidation. This assay is based on the reaction of MDA with a chromogenic agent thiobarbituric acid(TBA) at high temperature and acidic conditions to form MDA-TBA adduct(1:2). This complex has absorption maximum at 532nm and can be measured spectrophotometrically between 530 - 540nm. Absorbance is directly proportional to the TBARS concentration in the sample.

[CCK061] It is based on the reduction of nitrate(NO3-) to nitrite(NO2-) by a reducing agent at 37°C. Converted nitrite and endogenous nitrite are collectively converted by Griess reagent to a blue colored azo compound. This compound can be measured spectrophotometrically between 580-630nm and absorbance is directly proportional to the total nitric oxide concentration in the sample which is calculated from standard plot of NaNO3.

[CCK004] The EZBlue™ Cell Assay Kit is based on reduction of the water soluble, redox dye EZBlue™. The dye itself is dark blue in color and has intrinsic fluorescence. The fluorescence changes when EZBlue™ is reduced by metabolically active cells to the pink resorufin product. The spectral properties of resorufin allows the molecule to be detected using either absorbance or fluorescence.

[CCK070] The EZcountTM Homogenous MTS Cell Assay kit is designed for determination of cell viability and cell proliferation and/or effect of cytotoxic agent. This kit is based on the quantitative measurement of extracellular reduction of the water soluble tetrazolium dye MTS [2-(4-Iodophenyl)-3-4-nitrophenyl)-5-(2, 4 - disulfophenyl)-2H-tetrazolium sodium salt] to water soluble coloured formazan product by metabolically active cells.

[CCK051] The EZcountTM ATP Cell Assay Kit is designed for determination of cell viability and cell proliferation and/or effect of cytotoxic agents. The kit is based on the generation of luminescence as ATP catalyzes the reaction between D-luciferin and enzyme luciferase. ATP is ubiquitously present in all viable, metabolically active cells. On the other hand, cell death is characterized by a significant decrease in the ATP level.

[CCK031] The EZcountTM BrdU Cell Assay kit is designed for determination of cell proliferation and / or effect ofcytotoxic agent. When cells are cultured with labeling medium containing Pyrimidine analogue 5-bromo-2’-deoxyuridine(BrdU), BrdU gets incorporated in the DNA of proliferating cells in place of thymidine. BrdU labelled proliferating cells can be detected using Anti-BrdU antibody. The labeling medium is removed, and cells are fixed and DNA is denatured using fixation/denaturation solution to make BrdU accessible to the Anti-BrdU-HRP antibody. HRP substrate TMB is added for color development. The magnitude of the absorbance for the developed color directly correlates to the quantity of BrdU incorporated into cells, which is a direct indication of cell proliferation.

[CCK069] Glucos-6-Phosphate Dehydrogenase(G6PD) is a cytosolic enzyme present in all cells including RBCs. Plasma membrane of live cells is impermeable to G6PD. However, it is rapidly released into the culture medium when plasma membrane is damaged. The quantity of the released G6PD is used as a measure of cell cytotoxicity. CCK069 has been designed for rapid determination of cell death. The dye used in the assay is non-radioactive, water soluble and stable in most of the culture media.

[CCK058] CCK058 has been designed for rapid determination of cell cytotoxicity in a one step reaction. Non-toxicity of the LDH reagent allows performing the assay in a single culture vessel i.e. in homogenous format. The reagent can accurately determine cell cytotoxicity in the samples containing mixed population of viable and dead cells. This feature makes the method suitable for high throughput automation wherein minimum handling and manipulations are required.

[CCK036] Lactate dehydrogenase(LDH) is a cytoplasmic enzyme present in all mammalian cells. Plasma membrane of live cells is impermeable to LDH. However it is rapidly released into the culture medium when plasma membrane is damaged. CCK036 has been designed for rapid determination of cell cytotoxicity using an enzyme coupled two step reaction.

[CCK053] The EZcountTM MTS Cell Assay kit is designed for determination of cell viability and cell proliferation and/or effect of cytotoxic agent. This kit is based on the quantitative measurement of extracellular reduction of the water soluble tetrazolium dye MTS [2-(4-Iodophenyl)-3-4-nitrophenyl)-5-(2,4-disulfophenyl)-2H-tetrazolium sodium salt] to water soluble coloured formazan product by metabolically active cells.

[CCK003] The EZcountTM MTT Cell Assay kit is designed for determination of cell viability and cell proliferation and/or effect of cytotoxic agent. This kit is based on the quantitative measurement of extracellular reduction of the yellow colored water soluble Tetrazolium dye 3-[4, 5-dimethylthiazol-2-yl]-2, 5-diphenyl tetrazolium bromide(MTT) to insoluble formazan crystals by metabolically active cells.

[CCK034] The EZcountTM Neutral Red Cell Assay Kit is designed for determination of cell viability and cell proliferation and/or effect of cytotoxic agent. This kit is based on the quantitative measurement of uptake of neutral red by cellular lysosomes. Neutral red is a weak cationic dye that readily penetrates the cell membrane by non-ionic diffusion.