[CCK070] The EZcountTM Homogenous MTS Cell Assay kit is designed for determination of cell viability and cell proliferation and/or effect of cytotoxic agent. This kit is based on the quantitative measurement of extracellular reduction of the water soluble tetrazolium dye MTS [2-(4-Iodophenyl)-3-4-nitrophenyl)-5-(2, 4 - disulfophenyl)-2H-tetrazolium sodium salt] to water soluble coloured formazan product by metabolically active cells.

[CCK032] The EZcountTM WST-1 Cell Assay kit is designed for determination of cell viability, cell proliferation and cytotoxicity. This kit is based on the quantitative measurement of extracellular reduction of the water soluble tetrazolium dye WST-1 [2-(4-Iodophenyl)-3-4 -nitrophenyl)-5-(2,4-disulfophenyl)-2H-tetrazolium sodium salt] to a water-soluble coloured formazan product by metabolically active cells.

[CCK028] EZAssayTM GST Activity Estimation kit is simple and convenient tool for sensitive and reproducible detection and quantitation of GST activity in an array of samples like serum, cell lysates and tissue homogenates. This assay is based on the GST-catalyzed reaction between reduced glutathione and the GST substrate 1-chloro-2,4-dinitrobeneze(CDNB). CDNB can react with the broadest range of GST isoenzymes. The conjugation of glutathione to the CDNB substrate results in an increase in absorbance at 340nm.

[CCK004] The EZBlue™ Cell Assay Kit is based on reduction of the water soluble, redox dye EZBlue™. The dye itself is dark blue in color and has intrinsic fluorescence. The fluorescence changes when EZBlue™ is reduced by metabolically active cells to the pink resorufin product. The spectral properties of resorufin allows the molecule to be detected using either absorbance or fluorescence.

[CCK003] The EZcountTM MTT Cell Assay kit is designed for determination of cell viability and cell proliferation and/or effect of cytotoxic agent. This kit is based on the quantitative measurement of extracellular reduction of the yellow colored water soluble Tetrazolium dye 3-[4, 5-dimethylthiazol-2-yl]-2, 5-diphenyl tetrazolium bromide(MTT) to insoluble formazan crystals by metabolically active cells.

[TCL046] Trypan blue 0.4% Solution in Dulbecco’s Phosphate Buffered Saline

Net weight: 0,1 kg

[TCL005] Trypan blue 0.5% Solution in Dulbecco’s Phosphate Buffered Saline

Net weight: 0,1 kg

[TCL004] Phenol red is used as a pH indicator since its color exhibits a gradual change from yellow to red over a pH range of 6.8 to 8.2. Phenol red solution is widely used in cell culture for monitoring pH changes. The living tissues flourish in an environment of neutral pH. Hence it is required that the pH of the medium in which the cells are cultured must be maintained at near-neutral range. On acidification, the medium will turn yellow indicating the need of replacement of medium or it is also indicative of bacterial contamination. TCL004 is sterile filtered 0.5% Phenol red solution in Dulbecco's Phosphate Buffered saline.

Net weight: 0,1 kg

[CCK044] Microscopy Slide Set for Mammalian Cell lines
Contains a set of slides of five cell lines:
a) BHK-21
b) CHO
c) Vero
d) HEK-293
e) Sp2/O

[TCL115] Standard technique for staining reticulocytes is the new methylene blue staining. New methylene blue stains intracellular RNA blue in colour against pink stained RBCs. Depending on the stage of the reticulocyte the blue colour may be distributed in a general pattern(early reticulocytes) or punctate pattern(late reticulocyte). TCL115 is a reticulocyte staining solution formulated to contain new methylene blue for staining reticulocyte in blood smear.

Net weight: 0,1 kg

[TC255] Alizarin Red S

[TC707] Nile Red

Net weight: 0,0001 kg

[TC716] Acridine yellow

Net weight: 0,005 kg

[CCK008] The EZdetectTM Hoechst stain kit has been designed for detection of mycoplasma based on rapid uptake of the fluorescent stain Hoechst by cellular DNA. It selectively binds minor grooves of the DNA and hence allows for efficient staining of the nuclei. Hoechst stain can be excited either with a xenon mercury arc lamp or a UV laser and is detected through a blue filter. Mycoplasma staining with Hoechst appears as a fine particulate or filamentous staining over the cytoplasm at 100X magnification. Nuclei of the cells are also brightly stained by this method and thereby act as endogenous positive control for the staining procedure.

[CCK007] The EZdetectTM DAPI stain kit has been designed for detection of mycoplasma based on rapid uptake of the fluorescent stain DAPI by cellular DNA. DAPI selectively binds minor grooves of the DNA. The excitation maximum of dsDNA-bound DAPI is 358nm and emission maximum is 461nm. Mycoplasma staining with DAPI appears as a fine particulate or filamentous staining over the cytoplasm at 100X magnification. Nuclei of the cells are also brightly stained by this method and thereby act as endogenous positive control for the staining procedure.

[CCK034] The EZcountTM Neutral Red Cell Assay Kit is designed for determination of cell viability and cell proliferation and/or effect of cytotoxic agent. This kit is based on the quantitative measurement of uptake of neutral red by cellular lysosomes. Neutral red is a weak cationic dye that readily penetrates the cell membrane by non-ionic diffusion.

[TC356] New Methylene Blue N Zinc chloride double salt

[CCK072] This assay kit has been designed for measuring the ferric reducing ability of test sample. In this assay, ferric ion-chromogen complex gets reduced to colored ferrous ion at low pH. FRAP values are obtained by comparing the absorbance change at 593nm in test reaction mixtures with those containing ferrous ions in known concentration.

[CCK071] It is based on ability of antioxidants in the sample to reduce copper(II)-chromogen oxidant complex. Absorbance of the reduced copper(I)-chromogen complex is indicative of antioxidant capacity of the test sample.

[CCK078] EZAssayTM ROS Kit is designed for determination of reactive oxygen species activity within a cell using a cell permeable fluorogenic dye. Once fluorogenic dye diffuses inside the cells it gets deacetylated to non-fluorescent compound due to esterases. In presence of ROS, non fluorescent dye immediately gets oxidized. Fluorogenic dye is easily detectable using wavelength of excitation-488nm and emission-528nm. The fluorescent intensity produced is directly proportional to ROS level in the cells.