How to improve counting pollen:
Learn how CASY works with counting pollen
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A complete workflow was recently published by Kakui et al. and is available for download. CASY provides precise counts for pollen. Even more, CASY can distinguish between pollen of different size or between fully- and undeveloped pollen.
The CASY Cell Counter is a powerful tool for counting pollen numbers. We identified the first pollen-number controlling gene from Arabidopsis thaliana (model plant) by using CASY.
CASY was really helpful for our study because we needed to count a lot of smaples from different accessions and CRISPR-mutated invidiuals. The advantages of CASY are high-throughput measuring, reliability, and size distinction.
Alhtough pollen grains have a large variation among plant species, we confirmed CASY is able to count pollen grain numbers from a great variety of plant species (for example, Arabdiopsis thaliana, Arabidopsis kamchatica, wheat, Japanese cedar, Torenia fournieri, etc.). The CASY cell counter is a crucial tool for my research.
Dr. Hiroyuki Kakui, Graduate School of Science and Technology, Nigata University, Japan