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EZcountTM MTT Cell Assay Kit
60.31 60.31 60.31 60.31 USD
[CCK003] The EZcountTM MTT Cell Assay kit is designed for determination of cell viability and cell proliferation and/or effect of cytotoxic agent. This kit is based on the quantitative measurement of extracellular reduction of the yellow colored water soluble Tetrazolium dye 3-[4, 5-dimethylthiazol-2-yl]-2, 5-diphenyl tetrazolium bromide(MTT) to insoluble formazan crystals by metabolically active cells.

refrigerated goods (2°C - 8°C)
EZBlueTM Cell Assay Kit
623.54 623.54 623.54 623.54 USD
[CCK004] The EZBlue™ Cell Assay Kit is based on reduction of the water soluble, redox dye EZBlue™. The dye itself is dark blue in color and has intrinsic fluorescence. The fluorescence changes when EZBlue™ is reduced by metabolically active cells to the pink resorufin product. The spectral properties of resorufin allows the molecule to be detected using either absorbance or fluorescence.

refrigerated goods (2°C - 8°C)
EZkillTM Mycoplasma Elimination Kit
97.43 97.43 97.43 97.43 USD
[CCK006] EZkillTM Mycoplasma Elimination Kit is an antibiotic based elimination kit designed to eliminate mycoplasma from cell culture. Mycoplasma contamination of cell culture is a major problem in both research and industrial production. Ideally it is recommended to discard the contaminated culture after autoclaving. Only in cases where cell line is very important and non-replaceable, treatment for elimination should be considered. Antibiotic based method is the most common and effective method with no marked cytotoxicity.

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EZdetectTM DAPI Stain Kit for Mycoplasma Detection
55.67 55.67 55.67 55.67 USD
[CCK007] The EZdetectTM DAPI stain kit has been designed for detection of mycoplasma based on rapid uptake of the fluorescent stain DAPI by cellular DNA. DAPI selectively binds minor grooves of the DNA. The excitation maximum of dsDNA-bound DAPI is 358nm and emission maximum is 461nm. Mycoplasma staining with DAPI appears as a fine particulate or filamentous staining over the cytoplasm at 100X magnification. Nuclei of the cells are also brightly stained by this method and thereby act as endogenous positive control for the staining procedure.

refrigerated goods (2°C - 8°C)
EZdetectTM Hoechst Stain Kit for Mycoplasma Detection
53.82 53.82 53.82 53.82 USD
[CCK008] The EZdetectTM Hoechst stain kit has been designed for detection of mycoplasma based on rapid uptake of the fluorescent stain Hoechst by cellular DNA. It selectively binds minor grooves of the DNA and hence allows for efficient staining of the nuclei. Hoechst stain can be excited either with a xenon mercury arc lamp or a UV laser and is detected through a blue filter. Mycoplasma staining with Hoechst appears as a fine particulate or filamentous staining over the cytoplasm at 100X magnification. Nuclei of the cells are also brightly stained by this method and thereby act as endogenous positive control for the staining procedure.

refrigerated goods (2°C - 8°C)
EZdetectTM PCR kit for Mycoplasma detection Based on 16S-23S rRNA spacer region
94.55 94.55 94.55 94.55 USD
[CCK009] Mycoplasmas are the smallest known, simple prokaryotes, constituting one of the most widely spread contaminants found in cell culture. This kit has been designed for detection of mycoplasma based on amplification of spacer region between 16S and 23S rRNA genomic DNA sequence. This sequence is highly conserved between various mycoplasma species. This allows for detection of commonly encountered cell culture contaminants. Eukaryotic DNA is not amplified. A successful PCR reaction is indicated by a distinct band of 350-400bp derived from the internal control DNA on the agarose gel.

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EZcountTM XTT Cell Assay Kit
153.10 153.10 153.10 153.1 USD
[CCK015] The EZcountTM XTT Cell Assay kit is designed for determination of cell viability and cell proliferation and / or effect of cytotoxic agent. This kit is based on quantitative measurement of extracellular reduction of XTT to water soluble orange coloured formazan derivative by metabolically active cells.

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EZdetectTM PCR kit for Mycoplasma detection Based on 16S rRNA sequence
94.55 94.55 94.55 94.55 USD
[CCK022] Mycoplasmas are the smallest known, simple prokaryotes, constituting one of the most widely spread contaminants found in cell culture. PCR based detection of mycoplasma is one of the most convenient method. This kit has been designed for detection of mycoplasma based on amplification of 16S rRNA genomic DNA sequence. This sequence is highly conserved between various mycoplasma species. Eukaryotic DNA is not amplified. A successful PCR reaction is indicated by a distinct band of 502 - 520bp derived from the internal control DNA on the agarose gel.

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EZAssayTM TBARS Estimation Kit for Lipid Peroxidation
83.51 83.51 83.51 83.51 USD
[CCK023] Melondialdehyde(MDA) is one of such TBARS used for estimation of lipid peroxidation. This assay is based on the reaction of MDA with a chromogenic agent thiobarbituric acid(TBA) at high temperature and acidic conditions to form MDA-TBA adduct(1:2). This complex has absorption maximum at 532nm and can be measured spectrophotometrically between 530 - 540nm. Absorbance is directly proportional to the TBARS concentration in the sample.

refrigerated goods (2°C - 8°C)
EZAssayTM GST Activity Estimation Kit
88.15 88.15 88.15 88.15 USD
[CCK028] EZAssayTM GST Activity Estimation kit is simple and convenient tool for sensitive and reproducible detection and quantitation of GST activity in an array of samples like serum, cell lysates and tissue homogenates. This assay is based on the GST-catalyzed reaction between reduced glutathione and the GST substrate 1-chloro-2,4-dinitrobeneze(CDNB). CDNB can react with the broadest range of GST isoenzymes. The conjugation of glutathione to the CDNB substrate results in an increase in absorbance at 340nm.

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EZcountTM BrdU Cell Assay Kit
389.71 389.71 389.71 389.71000000000004 USD
[CCK031] The EZcountTM BrdU Cell Assay kit is designed for determination of cell proliferation and / or effect ofcytotoxic agent. When cells are cultured with labeling medium containing Pyrimidine analogue 5-bromo-2’-deoxyuridine(BrdU), BrdU gets incorporated in the DNA of proliferating cells in place of thymidine. BrdU labelled proliferating cells can be detected using Anti-BrdU antibody. The labeling medium is removed, and cells are fixed and DNA is denatured using fixation/denaturation solution to make BrdU accessible to the Anti-BrdU-HRP antibody. HRP substrate TMB is added for color development. The magnitude of the absorbance for the developed color directly correlates to the quantity of BrdU incorporated into cells, which is a direct indication of cell proliferation.

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EZcountTM WST-1 Cell Assay Kit
115.98 115.98 115.98 115.98 USD
[CCK032] The EZcountTM WST-1 Cell Assay kit is designed for determination of cell viability, cell proliferation and cytotoxicity. This kit is based on the quantitative measurement of extracellular reduction of the water soluble tetrazolium dye WST-1 [2-(4-Iodophenyl)-3-4 -nitrophenyl)-5-(2,4-disulfophenyl)-2H-tetrazolium sodium salt] to a water-soluble coloured formazan product by metabolically active cells.

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EZcountTM Neutral Red Cell Assay Kit
56.60 56.60 56.60 56.6 USD
[CCK034] The EZcountTM Neutral Red Cell Assay Kit is designed for determination of cell viability and cell proliferation and/or effect of cytotoxic agent. This kit is based on the quantitative measurement of uptake of neutral red by cellular lysosomes. Neutral red is a weak cationic dye that readily penetrates the cell membrane by non-ionic diffusion.
EZAssayTM Alkaline Phosphatase Activity Estimation Kit
154.95 154.95 154.95 154.95000000000002 USD
[CCK035] Alkaline phosphatase(ALP) is a group of isoenzymes that hydrolyze phosphate esters and generate inorganic phosphate at an alkaline pH. CCK035 is a simple colorimetric kit designed to detect the presenceof alkaline phosphatase activity.

refrigerated goods (2°C - 8°C)
EZcountTM Lactate Dehydrogenase Cell Assay Kit
96.49 96.49 96.49 96.49000000000001 USD
[CCK036] Lactate dehydrogenase(LDH) is a cytoplasmic enzyme present in all mammalian cells. Plasma membrane of live cells is impermeable to LDH. However it is rapidly released into the culture medium when plasma membrane is damaged. CCK036 has been designed for rapid determination of cell cytotoxicity using an enzyme coupled two step reaction.

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EZAssayTM Glutamine Glutamate Determination Kit
202.28 202.28 202.28 202.28 USD
[CCK037] L-glutamate is most abundant intracellular amino acid whereas L-glutamine is the most abundant extracellular amino acid. Estimation of L-glutamate and L-glutamine levels in the culture medium is thus indicative of the cellular metabolism and health of the cells. CCK037 is designed for estimation of concentration of these amino acids in cell culture samples such as cell lysates and spent media.

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Microscopy Slide Set for Mammalian Cell lines
20.41 20.41 20.41 20.41 USD
[CCK044] Microscopy Slide Set for Mammalian Cell lines
Contains a set of slides of five cell lines:
a) BHK-21
b) CHO
c) Vero
d) HEK-293
e) Sp2/O
EZcountTM ATP Cell Assay Kit
213.41 213.41 213.41 213.41 USD
[CCK051] The EZcountTM ATP Cell Assay Kit is designed for determination of cell viability and cell proliferation and/or effect of cytotoxic agents. The kit is based on the generation of luminescence as ATP catalyzes the reaction between D-luciferin and enzyme luciferase. ATP is ubiquitously present in all viable, metabolically active cells. On the other hand, cell death is characterized by a significant decrease in the ATP level.

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EZcountTM Sulphorhodamine B Cell Assay Kit
108.57 108.57 108.57 108.57000000000001 USD
[CCK052] The EZcount™ Sulphorhodamine B cell assay kit is designed for determination of cell viability and cell proliferation and/or effect of cytotoxic agents. This kit is based on the quantitative measurement of cellular protein.
EZcountTM MTS Cell Assay Kit
167.02 167.02 167.02 167.02 USD
[CCK053] The EZcountTM MTS Cell Assay kit is designed for determination of cell viability and cell proliferation and/or effect of cytotoxic agent. This kit is based on the quantitative measurement of extracellular reduction of the water soluble tetrazolium dye MTS [2-(4-Iodophenyl)-3-4-nitrophenyl)-5-(2,4-disulfophenyl)-2H-tetrazolium sodium salt] to water soluble coloured formazan product by metabolically active cells.

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