[SFF001P] GS-CHO Feed Supplement, Chemically Defined, Animal Component-Free w/ D-Glucose and Pluronic F-68® w/o L-Glutamine

[SFF002P] CHO-DHFR- Feed Supplement Chemically Defined, Animal Component-Free w/ D-Glucose and Pluronic F-68® w/o L-Glutamine and Phenol red

[SFF003P] CHO-K1 Feed Supplement, Chemically Defined, Animal Component-Free w/ D-Glucose and Pluronic F-68® w/o L-Glutamine

[SFM007AP] GS-CHO Serum Free CHO Medium, Animal Component Free, Chemically Defined w/ Pluronic F-68® w/o L-Glutamine and Sodium bicarbonate Part A - Basal Medium (powder)
Part B - Serum-free Growth Supplement

[SFM007L] GS-CHO Serum Free CHO Medium, Animal Component Free, Chemically Defined w/ Sodium bicarbonate and Pluronic F-68® w/o L-Glutamine Sterile filtered
1X Liquid Serum Free Cell Culture Medium Part A - Basal Medium (liquid)
Part B - Serum-free Growth Supplement

[SFM032AP] CHO-DHFR- Serum Free CHO Medium, Animal Component Free, Chemically Defined w/ Pluronic F-68® w/o L-Glutamine, Sodium bicarbonate and Phenol red Part A - Basal Medium (powder)
Part B - Serum-free Growth Supplement

Price for 10L on request

refrigerated goods (2°C - 8°C)

[SFM032L] CHO-DHFR- Serum Free CHO Medium, Animal Component Free, Chemically Defined w/Sodium bicarbonate and Pluronic F-68® w/o L-Glutamine and Phenol red Sterile filtered
1X Liquid Serum Free Cell Culture Medium Part A: Basal medium (Liquid)
Part B: Serum-free growth supplement

[SFM033AP] CHO-K1 Serum Free CHO Medium, Animal Component Free, Chemically Defined w/ Pluronic F-68® w/o L-Glutamine, Sodium bicarbonate and Phenol red Part A - Basal Medium (powder)
Part B - Serum-free Growth Supplement

Price for 5L on request

refrigerated goods (2°C - 8°C)

[SFM033L] CHO-K1 Serum Free CHO Medium, Animal Component Free, Chemically Defined w/ Sodium bicarbonate and Pluronic F-68® w/o L-Glutamine and Phenol red Sterile filtered
1X Liquid Serum Free Cell Culture Medium Part A - Basal Medium (liquid)
Part B - Serum-free Growth Supplement

[TCL023] Pluronic® F-68 is a co-polymer of ethylene and propylene oxide. It is a non-ionic detergent and is used as an anti-foaming agent in cell culture. It helps in stabilizing the cell membranes against the shear forces exerted during batch culture processes. It is suitable for insect cell culture. TCL023 is sterile filtered 10% Pluronic® F-68 solution.

Net weight: 0,1 kg

[TCL035] TCL035 is sterile filtered recombinant human insulin solution formulated to contain 10mg recombinant human insulin per ml.

Net weight: TCL035-20ML: 0,02 kg
TCL035-10ML: 0,01 kg

refrigerated goods (2°C - 8°C)

[TCL036] Bovine albumin Fraction V, also known as Fraction V is a protein derived from the serum of a cow. Fraction V gets its name "Cohn Fraction V" on account of its fractionation by the Edward Cohn purification methodology using cold ethanol precipitation. It also serves as protective agent against toxic metabolites.TCL036 is sterile filtered 7.5% albumin bovine fraction V solution in Dulbecco's Phosphate Buffered saline.

Net weight: 0,1 kg

refrigerated goods (2°C - 8°C)

[TCL064] ITS supplement is a basal growth factor supplement used to reduce the serum requirements in reduced serum and serum free cell culture systems.

Net weight: 0,01 kg

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[TCL065] ITS-E supplement is a basal growth factor supplement used to reduce the serum requirements in reduced serum and serum free cell culture systems.

Net weight: 0,01 kg

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[TCL066] ITS-P supplement is a basal growth factor supplement used to reduce the serum requirements in reduced serum and serum free cell culture systems.

Net weight: 0,01 kg

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[TCL072] HAT supplement contains Hypoxanthine, Aminopterin and Thymidine and is used for the preparation of selection medium for hybridoma. The myeloma cell is deficient in enzyme hypoxanthine-guanine phosphoribosyl transferase (HGPRT) or thymidine kinase (TK), and cannot survive in selection medium containing hypoxanthine, aminopterin and thymidine. TCL072 is a sterile filtered 50X HAT medium supplement containing 680.5 mg/litre Hypoxanthine, 8.8 mg/litre Aminopterin and 193.8 mg/litre Thymidine in Phosphate Buffered Saline.

Net weight: TCL072-100ML: 0,1 kg
TCL072-10X10ML: 0,1 kg
TCL072-5X10ML: 0,05 kg

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[TCL073] The specific action of aminopterin present in the HAT system is to inhibit the de novo DNA synthesis. Hence, it needs to be eliminated once the hybrids are selected and isolated. Aminopterin is diluted by several passages in hypoxanthine-thymidine (HT) supplemented medium for about 2-3 weeks. Once the de novo nucleoside-synthesis pathway has been reinstated, HT supplementation can be discontinued.
TCL073 is a sterile filtered, cell culture tested 50X HT medium supplement containing 680.5 mg/litre Hypoxanthine and 193.8 mg/litre Thymidine in Phosphate Buffered Saline.

Net weight: TCL073-100ML: 0,1 kg
TCL073-10x10ML: 0,1 kg
TCL073-5x10ML: 0,1 kg

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[TCL077] TCL077 is sterile solution formulated to contain 45% w/v glucose in tissue culture grade water. This solution is sterilized by autoclaving.

Net weight: TCL077-500ML: 0,5 kg
TCL077-100ML: 0,1 kg

[TCL088] The Glutamine Synthetase Expression Medium (GSEM) with supplement is a system that supports the growth of transfected cells containing the gene for the enzyme Glutamine synthetase. TCL088 is sterile-filtered, 50X liquid Glutamine Synthetase Expression Medium Supplement.

Net weight: 0,1 kg

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[TCL091] In serum supplemented cell cultures, serum serves as a major source of extracellular lipids. In serum-free cell culture systems, lipids are added in the form of lipid supplements or lipid concentrates. These are mixtures of different fatty acids, detergents and lipids that are essential for cell growth and maintenance in serum-free conditions.
TCL091 is a sterile filtered 100X lipid supplement formulated to contain chemically defined lipids and fatty acids.

Net weight: 0,1 kg

refrigerated goods (2°C - 8°C)